Beer's Law
What is the relationship between solute concentration and light absorption?
Try this
Using the Light Meter tool, measure the light intensity as it passes through various cuvettes filled with different known concentrations of colored soda (or other solute). Plot absorbance (as measured by log (I0/I), the log of the intensity of light as observed through a control cuvette filled with water divided by the intensity of light observed through a cuvette filled with the solution) versus concentration.
Challenge yourself
- Determine the mathematical relationship between absorbance and concentration.
- What is the meaning of the slope and y-intercept of the linear relationship observed?